Oil-based vitamin E oral spray for oral health in pregnancy

Sabrina Reppuccia, Felice Crocetto, Elisabetta Gragnano, Pietro D'Alessandro, Martin Vetrella, Gabriele Saccone, Bruno Arduino

Future Sci OA . 2022 Mar 1;8(4):FSO790. doi: 10.2144/fsoa-2021-0095. eCollection 2022 Apr.


Aim: To assess the efficacy of vitamin E oral spray in pregnancy.

Materials & methods: This was a retrospective study aimed to evaluate efficacy of vitamin E oral spray (vitamin E acetate in a medium chain tryglicerides vehicle – patented formulation) starting from the first trimester of pregnancy, with a control group.

Results: A total of 100 women were included in the study and were compared with a matched control group. Only 25/200 women reported to have at least one teeth cleaning during pregnancy. Women who received the oral spray had a significantly lower risk of preterm birth compared with the control group, and lower risk of periodontal diseases.

Conclusion: Use of oil-based vitamin E oral spray in pregnancy is associated with a decreased risk of periodontal diseases and therefore preterm birth.

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Vitamin E enriched diet increases the rate of orthodontic tooth movement

Christina Seong, Po-Jung Chen, Zana Kalajzic, Shivam Mehta, Ambika Sharma, Ravindra Nanda, Sumit Yadav, Eliane H Dutra

Am J Orthod Dentofacial Orthop . 2022 Jan 7;S0889-5406(21)00786-1. doi: 10.1016/j.ajodo.2020.10.033. Online ahead of print.


Introduction: Vitamin E is a popular antioxidant suggested to affect bone turnover. However, the effects of a vitamin E enriched diet on the rate of tooth movement are unknown. Therefore, this study aimed to evaluate tooth movement in rats receiving a vitamin E enriched diet. In addition, we examined bone remodeling in experimental and control rats.

Methods: Thirty-two 6-week-old male rats were divided into 4 groups: (1) group 1 (n = 8): orthodontic tooth movement (OTM) for 4 days + regular diet; (2) group 2 (n = 8): OTM for 14 days + regular diet; (3) group 3 (n = 8): OTM for 4 days + vitamin E diet; and (4) group 4 (n = 8) – OTM for 14 days + vitamin E diet. Maxillary alveolar bones and femurs of rats were analyzed by microcomputed tomography and histology.

Results: Rats fed a vitamin E diet presented an increased OTM rate at days 4 and 14. We found an increased number of osteoclasts and decreased bone volume in the vitamin E diet group at day 14 of OTM. In addition, there was increased expression of the microphthalmia-associated transcription factor in the alveolar bone of the vitamin E diet group. In contrast, there was no difference in bone remodeling in femurs or alveolar bone at the control side.

Conclusions: We found that an enriched vitamin E diet increases the rate of OTM in rats, suggesting that vitamin E may be useful as an avenue to accelerate OTM.

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Vitamin E supplementation reduces stress levels from orthodontic force in Wistar rats ( Rattus norvegicus)

Erliera Sufarnap, Syafruddin Ilyas, Ervina Sofyanti, Darmayanti Siregar, Yumi Lindawati, Trio Novalia, Henny Kurnianingsih

Saudi Dent J . 2021 Dec;33(8):912-916. doi: 10.1016/j.sdentj.2021.09.004. Epub 2021 Sep 13.


Background: Orthodontic tooth movement is mediated by the inflammation process. Inflammation induces pain and increases the level of cortisol hormone as it triggers stress. The aim of this research was to observe the effects of vitamin E (VE) supplementation in reducing stress levels from orthodontic force in Wistar rats (Rattus norvegicus).

Methods: Wistar rats (n = 56) were divided into two groups: group 1 as the control group, and group 2 as the experimental group (VE group). VE supplemented for 14 days prior application of the separator as an orthodontic force. Each group was divided into four subgroups (n = 7), corresponding to the duration in days that force was applied, i.e., 0, 1, 3, and 7 days. Stress were measured by cortisol levels, and inflammation were measured by interleukin-1 beta (IL-1β) levels in blood plasma.

Results: The VE group had lower cortisol levels than the control group, and significant found on days 3 and 7 (p = 0.026 and p = 0.037). The cortisol level in the VE group decreased faster, beginning on day 1, whilst the control group occurred on day 3. Statistical analysis of IL-1β levels found insignificant differences between the two groups.

Conclusion: Vitamin E helps reduce stress caused by orthodontic force due to tooth movement.

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Vitamin E: A potential preventive approach against dental erosion-an in vitro short-term erosive study

Daniela Rios , Ana Paula Boteon, Camilla Cristina Lira Di Leone, Tainara Tonon Castelluccio, Fernanda Lyrio Mendonça, Franciny Querobim Ionta, Marília Afonso Rabelo Buzalaf, Thiago Saads Carvalho

J Dent . 2021 Oct;113:103781. doi: 10.1016/j.jdent.2021.103781. Epub 2021 Aug 13.


Objectives: This study evaluated the in vitro effect of different components of palm oil on enamel in a short-term erosive challenge.

Methods: The acquired enamel pellicle (AEP) was previously formed in situ for 2 h. Subsequently, the bovine enamel blocks were treated in vitro according to following solutions: G1-palm oil; G2-85% tocotrienol solution; G3-oily vitamin E; G4-oily vitamin A; G5-deionized water (negative control); G6-stannous-containing solution (Elmex® Erosion Protection Dental Rinse) (positive control). After application of the treatment solutions (500 µl, 30 s), the blocks were immersed in 0.5% citric acid (pH 2.4) during 30 s (initial erosion). The response variable was the percentage of surface hardness loss. Data were analyzed by one-way ANOVA and Fisher’s Test (p < 0.05).

Results: The positive control (G6), palm oil (G1) and oily vitamin E (G3) groups presented the lowest percentage of surface hardness loss, and were statistically different from the negative group (G5) (p < 0.05), and no differences were found between these three groups. The 85% tocotrienol solution (G2) and oily vitamin A groups (G4) were not different to the negative control group.

Conclusions: Stannous-containing positive control (Elmex® Erosion Protection), palm oil and oily Vitamin E were able to protect enamel against the erosive challenge performed in this in vitro study. In addition, vitamin E is probably the key ingredient of palm oil responsible for preventing enamel erosion.

Clinical significance: Vitamin E presented similar preventive effect to a commercial mouthwash stannous-containing solution (Elmex® Erosion Protection) against initial erosion and, it can be considered as a promising natural alternative for the formulations of solutions aiming to prevent erosive tooth wear.

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The effect of Vitamin E supplementation on treatment of chronic periodontitis

Parichehr Behfarnia, Mina Dadmehr, Seyedeh Negin Hosseini, Seyed Amir Mirghaderi

Dent Res J (Isfahan) . 2021 Aug 18;18:62. eCollection 2021.


Background: The purpose of this study was to evaluate the effect of Vitamin E supplements on chronic periodontitis based on the clinical parameters of pocket depth and clinical attachment level and total antioxidant capacity (TAC) of saliva.

Materials and methods: In this clinical trial, 16 patients with chronic periodontitis were selected and divided into two groups. The indices of pocket depth and attachment loss for 6 teeth per person were measured with a periodontal probe. A total of 41 teeth in the control group and 42 teeth in the case group were examined. Then, 2 ml nonstimulated saliva was collected from each patient. All patients were treated with scaling and root planing (SRP). The case group consumed 200 IU supplementary Vitamin E daily for up to 2 months. After 2 months, clinical indices were re-measured and 2 ml nonstimulated saliva was collected. The TAC of saliva samples was measured by using Zellbio’s TAC Kit. Data were analyzed by the SPSS software and were evaluated in each group between the first session and 2 months later with paired t-test. The differences between the two groups were evaluated through the independent t-test (α ≤ 0.05).

Results: Independent t-test showed that mean change in TAC (P = 0.14) and pocket depth changes (P = 0.33) was not significant between two groups 2 months after SRP, but mean attachment loss changes in the case group was significantly less than the control group (P = 0.03).

Conclusion: The results of this study indicate that Vitamin E supplementation with SRP can reduce the inflammatory process of periodontitis and improve periodontal clinical indices and decrease the amount of attachment loss.

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Effect of vitamin E on periodontitis: Evidence and proposed mechanisms of action

Saminathan Shadisvaaran, Kok-Yong Chin, Mohd-Said Shahida, Soelaiman Ima-Nirwana, Xin-Fang Leong

J Oral Biosci . 2021 Jun;63(2):97-103. doi: 10.1016/j.job.2021.04.001. Epub 2021 Apr 20.


Background: Periodontitis is a noncommunicable inflammatory disease of the soft tissue and bone surrounding the teeth in the jaw, which affects susceptible individuals with poor oral hygiene. A growing interest has been seen in the use of dietary supplements and natural products for the treatment and prevention of periodontitis. Vitamin E consists of two major groups, namely tocopherols and tocotrienols, which are botanical lipophilic compounds with excellent anti-inflammatory and antioxidant properties.

Highlight: This review aimed to summarize the preclinical and clinical findings on the effects of vitamin E on periodontitis. The current literature suggests that vitamin E could improve the periodontal status by correcting redox status imbalance, reducing inflammatory responses, and promoting wound healing, thus highlighting the potential of vitamin E in the management of periodontitis.

Conclusion: Direct evidence for the use of vitamin E supplementation or treatment of periodontitis in humans is still limited. More well-designed and controlled studies are required to ascertain its effectiveness.

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Systemic treatment with alpha-tocopherol and/or sodium selenite decreases the progression of experimental periodontitis

Nurgül Bas, Nezahat Arzu Kayar, Z Füsun Baba, Mustafa Cihat Avunduk, Seyfullah Haliloğlu, Nilgün Özlem Alptekin

Clin Oral Investig . 2020 Sep 28. doi: 10.1007/s00784-020-03579-9. Online ahead of print.


Objective: To investigate the effects of sodium selenite (Se) and/or α-tocopherol (αT) applications on the alveolar bone loss (ABL), the number of gingival collagen fibers, inducible nitric oxide synthase (iNOS)+ and CD95+ cell numbers, and serum cytokine concentrations in experimental periodontitis in rats.

Materials and methods: Forty Sprague Dawley rats were divided into four groups of ten as follows: group A: Se group, group B: αT group, group C: Se and αT combined group, and group D: control group (intraperitoneal (IP) saline injection applied). Using the image analysis method in the connective tissue under the connective epithelium, the numbers of iNOS, CD95 positive cells, and collagen fibers were counted. ELISA kits were used to test the concentrations of serum interleukin (IL)-1β, IL-6, and IL-4.

Results: The combination of Se and αT (group C) suppressed ABL compared with the control group (group D) (P < 0.05). In group A (Se), the number of iNOS+ cells was smaller than in group D (P < 0.05).

Conclusion: Se has been concluded to inhibit inflammation of the gum due to iNOS. Se and αT can have a remarkable important role in preventing alveolar bone loss, and particularly in combination.

Clinical relevance: Se and/or αT application may be useful in preventing the destruction of periodontal tissue and treatment of periodontal disease.

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Evaluation of the effects of vitamins C and E on experimental orthodontic tooth movement

Esra Bolat, Elçin Esenlik, Meral Öncü, Meltem Özgöçmen, Mustafa Cihat Avunduk, Özlem Yüksel

J Dent Res Dent Clin Dent Prospects . Spring 2020;14(2):131-137. doi: 10.34172/joddd.2020.0027. Epub 2020 Jun 17.


Background. This experimental study aimed to assess the effects of Vitamins C and E on orthodontic tooth movement. Methods. Fifty-one male Wistar albino rats were divided into six groups: five appliance groups and one control group. The appliance groups had an orthodontic appliance consisting of a closed-coil spring ligated between the maxillary incisor and maxillary first molar (50 g). Vitamin E and C (150 mg/kg) were injected intraperitoneally per day in the first and second groups, respectively. Vitamins E and C (20 μL) were locally injected into the periodontal gap of the moving teeth in the third and fourth groups, respectively, once every three days. No vitamin was injected in the last (fifth) appliance group.The experimental period was 18 days. Histological and biochemical (alkaline phosphatase, osteocalcin, and NTx levels) evaluations of the samples were performed, and maxillary incisor‒molar distance was measured before and after the experiment. Results. The amount of tooth movement was similar in the appliance groups. All the vitamin groups showed significantly increased osteoblastic activity, while those treated with systemic vitamins exhibited significantly increased numbers of collagen fibers on the tension side compared to the appliance control group (P<0.05). Conclusion. Vitamin C and E supplements positively affected bone formation on the tension side of the teeth during experimental orthodontic tooth movement.

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Interaction between and impact of IL-6 genotype and alpha-tocopherol levels on periodontal condition in aging individuals

Akihiro Yoshihara, Noboru Kaneko, Akane Miyamoto, Kaname Nohno

J Periodontal Res . 2020 Sep 20. doi: 10.1111/jre.12802. Online ahead of print.


Background and objectives: Few studies have assessed the possible interaction between and impact of IL-6 variants and serum α-tocopherol levels on periodontal condition in older individuals. Here, we assessed the relationship between IL-6 variants and serum α-tocopherol levels on periodontal condition by considering effect modification.

Material and methods: Among the study participants, 359 who were 71 years of age underwent a dental examination, biochemical analysis, and interview. After dividing the participants into tertiles based on serum α-tocopherol levels, we conducted Poisson regression analysis to compare the prevalence rate ratio (PRR) for periodontal disease markers with the IL-6 genotype (rs1800796) based on each tertile adjusted by the number of teeth present (offset).

Results: The PRRs of the IL-6 genotype for periodontal condition (probing pocket depth [PPD], clinical attachment level [CAL], and bleeding on probing [BOP]) which were adjusted by the number of teeth present (offset) were 1.17 (P < .001), 1.37 (P < .001), and 1.08 (P = .048), respectively. In addition, a significant association was found between the reciprocal number of PRRs of the IL-6 genotype and three serum α-tocopherol levels. The adjusted PRRs (± standard error) of the IL-6 genotypes for PPD were 0.48 (0.12) for the first group (P < .001), 1.54 (0.04) for the second group (P < .001), and 2.11 (0.03) for the third group (P < .001); similar tendencies were seen for CAL and BOP.

Conclusion: The results of this study suggest a potential association between the IL-6 genotype and periodontal condition in relation to serum antioxidant concentrations.

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