Tocol composition was studied in 26 genotypes of wheat grown in one location for three years and in three other locations for one year. Special emphasis was placed on evaluating the variation of tocols within wheat genotypes and among various growing conditions. In general, both genetic and environmental effects had a strong impact on tocols in wheat genotypes. Because the growing locations and years differed considerably, greater variation due to the environment was found in this study than in earlier ones. Some of the genotypes were more sensitive to the impact of the environment, whereas others were relatively stable. Of the wheat genotypes with an average total tocol content of >or=55 microg/g of dry matter, five genotypes showed relatively low variation: Claire, Cadenza, Lynx, Atlas 66, and Disponent. These genotypes could be potential candidates for the breeding of stable and high-tocol content wheat cultivars.