Monthly Archives: August 2021

Supplementation of antihypertensive drug regimen with vitamin E ameliorates alterations of primary haemodynamic parameters and total antioxidant capacity in ovariectomised rats

Temitayo Olabisi Ajibade, Foluso B Bolaji-Alabi, Ademola Adetokunbo Oyagbemi, Ifeoluwa W Ajileye, Temidayo Olutayo Omobowale

J Basic Clin Physiol Pharmacol . 2021 Aug 6. doi: 10.1515/jbcpp-2020-0097. Online ahead of print.

Abstract

Objectives: Ovariectomy induces heightened response to vasoconstrictors, alters vasorelaxation and consequently causes hypertension due to increased oxidative stress in rats.

Methods: This study evaluated the ameliorative effects of ramipril and vitamin E, on primary haemodynamic parameters and cardiac antioxidant defence status, in ovariectomised rats using 64 adult female rats of the Wistar strain randomly divided as follows: Control (sham); Ovariectomised (OVX); OVX plus Ramipril; OVX plus vitamin E; and OVX plus Ramipril plus vitamin E.

Results: The plasma level of oestrogen was significantly lower (p<0.05), in the ovariectomised rats compared with the sham. The systolic, diastolic and mean arterial blood pressure of ovariectomised rats increased significantly (p<0.05), but the alteration was significantly reduced by the administration of ramipril alone or in combination with vitamin E. Significant decrease (p<0.05) was observed in the serum level of nitric oxide in OVX group compared with Sham. Also, analysed markers of oxidative stress: Malondialdehyde (MDA) contents and hydrogen peroxide (H2O2) generated decreased significantly (p<0.05), but systemic antioxidants: reduced glutathione (GSH) contents; glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities increased significantly (p<0.05) in the ovariectomised rats treated with ramipril and vitamin E compared with untreated ovariectomised rats. The study concludes that alteration, in the primary haemodynamic parameters, associated with ovariectomy in rats is potently ameliorated by co-administration of the antihypertensive drug ramipril and vitamin E.

Conclusions: The supplementation of antihypertensive regimen with antioxidants such as vitamin E in the treatment of hypertension is therefore justifiable especially in ovariectomised or hypogonadal patients.

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Effects of Co-administration of Vitamin E and Lithium Chloride on Chronic Constriction Injury-induced Neuropathy in Male Wistar Rats: Focus on antioxidant and anti-inflammatory mechanisms

Kingsley Dominic Esu, Ahmed Olalekan Bakare, Bamidele Victor Owoyele

Pain Pract . 2021 Aug 5. doi: 10.1111/papr.13064. Online ahead of print.

Abstract

Objectives: This study investigated the antinociceptive effects of co-administration of lithium chloride (LiCl) and vitamin E (Vit. E) on chronic constriction injury (CCI)-induced peripheral neuropathy in male Wistar rats. It further explored the anti-inflammatory and neuroprotective properties of LiCl and Vit. E which may be complementary to the antinociceptive effects of the two substances.

Methods: Thirty-six male Wistar rats, 190.00 ± 10.00 g of body weight (b.w) were randomly assigned to six experimental groups and administered with either normal saline, Vit. E, LiCl, or their combination, once daily for twenty-one (21) days. CCI was used to induce NP and mechanical allodynia was assessed using von Frey filaments and pinprick test. Open field maze (OFM) was used to assess the exploratory behaviour. Biochemical parameters were assessed in the dorsal root ganglion (DRG) after twenty-one days of treatment.

Results: Mechanical allodynia was developed in rats following CCI. Co-administration of LiCl and Vit.E. synergistically reduced mechanical hyperalgesia in rats which were significantly different compared with the single administration of either Vit.E. or LiCl. Combined doses of Vit.E. and LiCl significantly increases the explorative behaviour in the OFM. CCI increased malondialdehyde (MDA), tumour necrotic factor-alpha (TNF-α), calcitonin gene-related polypeptide (CGRP), calcium ion (Ca2+ ), and reduced superoxide dismutase (SOD) activities. Co-administration of LiCl and Vit.E. significantly reduced MDA, TNF-α, but increased SOD compared with ligated control.

Discussion: The findings revealed that the synergistic effects of the co-administration of Vit. E and LiCl in ameliorating neuropathic pain are mediated by their anti-inflammatory and antioxidant properties.

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Vitamin E-facilitated carbon monoxide pro-drug nanomedicine for efficient light-responsive combination cancer therapy

Yaw Opoku-Damoah, Run Zhang, Hang T Ta, Zhi Ping Xu

Biomater Sci . 2021 Aug 4. doi: 10.1039/d1bm00941a. Online ahead of print.

Abstract

The quest to maximize therapeutic efficiency in cancer treatment requires innovative delivery nanoplatforms capable of employing different modules simultaneously. Combination therapy has proven to be one of the best anticancer strategies so far. Herein, we have developed a lipid-encapsulated nanoplatform that combines chemotherapy with photoresponsive gas therapy for colon cancer treatment. Carbon monoxide releasing molecules (CORMs) and vitamin E analogues (pure/pegylated α-tocopheryl succinate; α-TOS) were co-loaded into the lipid layer with core-shell upconversion nanoparticles (UCNPs), which converted 808 nm light to 360 nm photons to trigger CO release at the tumor site. This folic acid (FA)-targeting nanomedicine (Lipid/UCNP/CORM/α-TOS/FA: LUCTF) possessed a cancer-targeting ability and a light-triggered CO release ability for synergistic apoptosis of HCT116 cells via enhanced ROS generation and mitochondrial membrane breaking. In vivo data have confirmed the significantly enhanced therapeutic efficacy of LUCTF without any significant biosafety issues after intravenous administration. Thus, nanomedicine LUCTF represents a novel way for efficient cancer therapy via combining locally released CO and a compatible chemotherapeutic agent (e.g. α-TOS).

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High cholesterol diet activates ER stress mediated apoptosis in testes tissue: Role of α-tocopherol

Erdi Sozen, Tugce Demirel-Yalciner, M Kutay Koroglu, Merve Acikel Elmas, Feriha Ercan, Nesrin Kartal Ozer

IUBMB Life . 2021 Aug 4. doi: 10.1002/iub.2535. Online ahead of print.

Abstract

The seminiferous tubules where spermatogenesis occurs are enveloped and protected by the Sertoli cells to support germ cells undergoing meiosis to produce haploid gametes. Clearly, induction of apoptosis in seminiferous tubules leads to abnormalities in spermatogenesis and male infertility. Studies demonstrated that increased hyperlipidemia impairs male infertility and spermatogenesis by enhancing seminiferous tubules apoptosis. However, molecular mechanisms underlying high-cholesterol-mediated testicular damage remain poorly elucidated. In this scope, we established a rabbit model and investigated the role of endoplasmic reticulum (ER) stress on high cholesterol diet induced seminiferous tubule apoptosis. Histopatological examinations revealed increased seminifer tubule apoptosis in testes of rabbits fed high cholesterol diet. In addition, phosphorylated forms of IRE1 and PERK, two well-identified markers of ER stress, were significantly induced in accordance with high cholesterol diet. High cholesterol diet also exhibited CHOP induction in testes, indicating increased ER stress related apoptosis. Supplementation of α-tocopherol significantly attenuated cholesterol mediated ER stress, and restored seminiferous tubules apoptosis. Taken together, our findings suggest that α-tocopherol might be capable to reduce testicular damage via ameliorating histopatological features and inhibiting seminiferous tubules apoptosis in hypercholesterolemic rabbits.

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The underrated antioxidant to hydrate weak, brittle nails

When it comes to nails, one has to be careful with the type of nail polish they put. A high-quality liquid is important and protecting the nails from becoming soft is certainly vital for its growth.

es, as much as your skin needs hydration from time to time, even your nails need to be moisturised to keep them healthy and shiny. The one ingredient that is an antioxidant that protects your cuticles is vitamin E or moisture-rich tocopherol, which is a fat-soluble vitamin. It helps to even out the nail plate (the hard part of the nail).

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Is the lower serum level of vitamin E associated with pregnant women with allergic rhinitis?

Yiu-Tai Li, Wen-Ling Lee, Peng-Hui Wang

J Chin Med Assoc . 2021 Aug 1;84(8):739-740. doi: 10.1097/JCMA.0000000000000566.

Abstract

It is well known that adequate maintenance or support of nutrition during pregnancy, including essential and trace elements and calorie intake, is a critical dimension not only to maintain peak health and performance of themselves but also for the lifelong health of the offspring.1–7 Malnutrition often results in inadequate protein intake, fewer calories, and deficiency of certain-type essential trace elements. Intake of enough calorie can be easily monitored by measuring gestational weight gain (GWG) in the entire pregnancy period or more accurately estimated by separating GWG according to the different trimesters3; however, it is hard to define whether these pregnant women have adequate dietary intake or meet recommendations for vitamins D, C, A, B complex, K, and E, as well as folate, choline, iron, calcium, potassium, magnesium, zinc, and other essential trace elements (minerals or essential amino acids and so on), partly because of difficulty to measure and monitor these essential trace elements, and partly because of overlooking its important and critical role on both maternal and offspring’s outcome.1,2,4–6 Additionally, these certain-type essential trace elements sometimes make physicians or healthcares confused, based on the presence of multifaced functions of these elements. It has been reported that continuous supplementation of vitamin E throughout offspring lifespan provides beneficial effects to the offspring, but one meta-analysis using experimental models and observational investigations, which are involved with more than 135 000 participants in 19 trials carried out between 1966 and 2004 did not support the above-mentioned findings, based on a significantly increasing mortality from all causes when high dosage vitamin E supplements are given as a supplement throughout their lifespan.

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Vitamin E Toxicity

Kristen N. Owen, Olga Dewald

In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021 Jan. 2021 Aug 1.

Excerpt

Vitamin E is a major lipid-soluble antioxidant obtained exclusively from the diet. It was discovered in the 1920s as an essential dietary element required by rats for reproduction. There are approximately eight different vitamin E-related molecules, but the dominant molecule in humans is alpha-tocopherol. Tocotrienols are the other molecules that are widely studied for vitamin E supplementation. These two molecules have been studied in various dosages and for many different health purposes. Vitamin E has peroxyl radical scavenger properties. While vitamin E toxicity is associated with an increased risk of bleeding, its deficiency has been associated with neurologic diseases and anemia.

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